In the present study, Dissimilar welding between Inconel 718 nickel base superalloy and 310S austenitic stainless steel using gas tungsten arc welding process was performed in order to microstructural characterization and weldability assessment of filler metal used. For this purpose, three filler metals including Inconel 625, Inconel 82 and 310 stainless steel were used. After welding, the microstructure of different regions including base and weld metals were metallographically characterized using the optical and scanning electron microscope equipped with energy dispersive spectroscopy. Moreover, to estimate susceptibility to solidification cracking of weld metals, Varestraint tests were used. Microstructural observations showed that solidification conditions have created a dendritic structure for Inconel 625 weld metal. Moreover, microstructure of the 310 weld metal was consisted of solidification micro-cracks that distributed along the solidification sub grain boundaries. Varestraint test also determined that, at all applied strain, Inconel 82 weld metal has the lowest susceptibility to solidification cracking among the weld metals. Finally, by comparing the results obtained, Inconel 82 filler metal was diagnosed the best choice for mentioned Dissimilar joint.